Environmental Applications of Biomolecular Engineering

Course content

Problems posed by natural and engineered environments for monitoring microorganisms; advantages and pitfalls of molecular techniques for microbial community analyses, FAME, PCR, 16S/18S rRNA sequencing, phylogenetic analysis and relationship between phylogenetic information and ecological function of the microbial communities; profiling of complex microbial populations by DGGE, TTGE, SSCP, RAPD, ARDRA, T-RFLP, LH-PCR, and RISA; enumeration, identification and monitoring of pollutant-degrading bacteria by using nucleic acid probes, FISH, MAR and SIP; omic technologies and post-genomic approaches, applications, applications of these biomolecular methods in environmental engineering.